Figure 2. BAF212 increases survival in mice after primary infection with WT C.neoformans H99.
(A) Mice received compound daily via gavage (FTY720, n = 8; BAF312, n = 8; or vehicle control [H2O], n = 8) starting 2 days before intranasal infection with C. neoformans H99 and survival of mice was monitored. Survival curves were compared using the log-rank (Mantel-Cox). **P = 0.0083. (B) After 40 days of daily compound administration, BAF312 mice that survived were euthanized and analyzed for CFUs in the lung and the brain, n = 4. All error bars represent SEM. (C) NBD-FTY720 was added to the media of either mammalian cells (J774A.1) or C. neoformans cells (Δgcs1 and H99) at a concentration of 2 μg/mL. NBD-FTY720 was extracted from the media and evaluated via thin layer chromatography, n = 3 independent experiments.