Figure 3. FTY720 and BAF312 both cause a decrease in T cells and innate immune cells in the lung and blood.

Mice were infected for 30 days with C. neoformans Δgcs1 before FTY720, BAF312, or vehicle control (H₂O) administration. For the blood analysis, n = 4 mice per group at each time point and for lungs, n = 4 mice per group at each time point (1 day before compound administration [day –1], day 5, and day 20 after compound administration). Experiment was conducted 2 times. For lung samples, leukocytes were labeled intravascularly to distinguish between cells in the lung parenchyma and vasculature before staining to identify immune cell populations. Statistical significance was determined using 2-way repeated measures ANOVA with Bonferroni’s multiple comparisons post hoc test. P values were corrected for multiplicity using the Bonferroni’s adjustment. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 compared with the control. Adjusted P values available in Supplemental Table 1.