Figure 5. Treatment with FTY720 but not BAF312 affects macrophage organization in the granuloma structure.

(A) Mice were infected for 30 days with C. neoformans Δgcs1 before FTY720, BAF312, or vehicle control (H₂O) administration. At day 60 after compound administration lungs were processed for F4/80 immunohistochemistry and Verhoeff–Van Gieson (VVG) staining. Scale bars: 200 μm (top), 50 μm (middle and bottom). Black box indicates enlarged area. The dashed line indicates the fibrotic granuloma layer identified by the red collagen and black elastin staining in VVG. (B) The mean intensity of F4/80 staining inside and outside the bounds of the granuloma, as delineated by the collagen deposition seen in VVG, was quantified using Image J software, n = 4. All error bars represent SEM. Comparisons were done with 1-way ANOVA with Bonferroni’s multiple comparisons post hoc test. P value was corrected for multiplicity using the Bonferroni’s adjustment. **P = 0.0021 compared with control.