Figure 7. FTY720 impairs phagocytosis and ROS in macrophages.

(A) A schematic of pathways downstream of S1PR3 in macrophages as well as the reported activity of the compounds used in C and D is shown. (B) Primary alveolar macrophages isolated from WT mice were treated overnight with 1 nM of indicated compound, n = 3. Cells were subsequently coincubated with opsonized C. neoformans Δgcs1 and phagocytic index was calculated by microscopic observation. Experiment was conducted 3 times. (C) Primary alveolar macrophages isolated from WT mice were treated with indicated compound for 1 hour, n = 3 each. Phagocytic index was performed as in B. Experiment was conducted 3 times. (D) Primary alveolar macrophages from WT mice were treated overnight with the respective compound and analysis of ROS production was performed, n = 3. Experiment was conducted 3 times. All error bars represent SEM and statistical comparisons were done using the 2-sided Student’s t test (B: *P = 0.0313) or 1-way ANOVA with Bonferroni’s multiple comparisons post hoc test. P values were corrected for multiplicity using the Bonferroni’s adjustment (C: ***P = 0.0098, ****P < 0.001, *****P < 0.0001; D: **P = 0.0218).