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. 2020 Jul 27;130(9):4561–4573. doi: 10.1172/JCI134778

Figure 5. Effect of β-glucan on K+ efflux and mtROS.

Figure 5

(A) Monocytes were preincubated or not with β-glucan and differentiated with either GM-CSF or M-CSF. Macrophages were primed for 3 hours with LPS, stained with APG-2, and then stimulated for 20 minutes with nigericin or vehicle as a control. Results are expressed as the average K+ content (average APG-2 fluorescence intensity per cell), as measured through Icy software’s image analysis pipeline. Data represent average values ± SEM of the analysis of 3 independent experiments. n = 7 and n = 9. *P < 0.05 and **P < 0.01, by 1-way ANOVA Friedman test with Dunn’s correction for multiple comparisons. (B) Macrophages were primed for 3 hours with LPS and then stimulated for 30 minutes with nigericin. Cells were stained with MitoSOX for mtROS levels. Data are expressed as the MFI relative to unstained cells. Ratio ± SEM of the analysis of 3 independent experiments. n = 4 (GM-CSF) and n = 3 (M-CSF). *P < 0.05, by paired, 2-tailed Student’s t test.