Table 1.

Kinetic parameters extracted from AFM-SMFS.

Pathway	Event	k0 [s−1] (BE)	Δx‡ [nm] (BE)	ΔG‡ [kBT] (DHS)		k0 [s−1] (DHS)		Δx‡ [nm] (DHS)
				υ = 0.5	υ = 2/3	υ = 0.5	υ = 2/3	υ = 0.5	υ = 2/3
1	High force rupture (XMod intact)	4.70 × 10−8	0.178	22.8	20.0	9.39 × 10−5	1.57 × 10−4	0.146	0.132
2	Unfolding of XMod (measured)	9.94 × 10−6	0.139	27.6	24.0	5.74 × 10−7	4.79 × 10−6	0.209	0.168
2	Unfolding of XMod (corrected)	4.53 × 10−5	0.116	30.1	26.1	2.66 × 10−6	5.42 × 10−6	0.173	0.157
2	Low force rupture after XMod unfold	7.43 × 10−4	0.277	13.9	12.5	0.0152	0.101	0.254	0.171
3	Low force rupture (XMod intact)	2.79 × 10−5	0.366	8.46	7.45	0.831	1.08	0.131	0.113