Figure 5. IRKO^GFAP mice show alterations in BAT morphology and larger droplet size at 9 months of age.

(A-B) Histological H&E analysis in females and males. (C) Quantitative analysis of fat droplet area in female and male mice. (D) Quantification of BAT cell counts in females and males. n=3 per group. Female IR^loxp group is represented with black bar/circle dots, female IRKO^GFAP group with dark grey bar/triangle dots, male IR^loxp group with black bar/circle dots and male IRKO^GFAP group with dark grey bar/triangle dots. Values are expressed as means ± SEM. * P<0.0332, * * P<0.0021, *** P<0.0002, **** P< 0.0001 by two-way ANOVA and Tukey post-hoc test relative to IRKO^GFAP versus IR^loxp groups.