Figure 7. IRKO^GFAP mice show less innervation of BAT tissue at 9–10 months of age.

(A-B) Representative images of immunohistochemical (IHC) sections of unstained and stained tyrosine hydroxylase (TH) in BAT tissues of females and males. (C) Quantitative analysis of positive nerve terminals in females and males. n=3 per group. The female IR^loxp group is represented with a black bar/circle dots, the female IRKO^GFAP group with dark a grey bar/triangle dots, the male IR^loxp group with a black bar/circle dots and the male IRKO^GFAP group with a dark grey bar/triangle dots. Values are expressed as means ± SEM. * P<0.0332, * * P<0.0021, *** P<0.0002, **** P< 0.0001 by two-way ANOVA and Tukey post-hoc test relative to IRKO^GFAP versus IR^loxp groups.