Fig. 3.

Defect in short- and long-term engraftment of Nlrp3-KO HSPCs in WT mice

Panel A. Lethally irradiated WT mice (9 per group) were transplanted with bone marrow mononuclear cells (BMMNCs) from WT or Nlrp3-KO mice, which had been previously labeled with a PKH67 cell linker. Twenty-four hours after transplantation, the femoral BMMNCs were harvested, the number of PKH67⁺ cells evaluated by FACS, and the CFU-GM clonogenic progenitors enumerated in an in vitro colony assay. Panel B. Lethally irradiated WT mice (9 per group) were transplanted with BMMNCs from WT or Nlrp3-KO mice, and 12 days after transplantation femoral BMMNCs were harvested and plated to count the number of CFU-GM colonies and the spleens removed for counting the number of CFU-S colonies. No colonies were formed in lethally irradiated, untransplanted mice (irradiation control). *p < 0.05. Panel C. Lethally irradiated mice (9 per group) were transplanted with BMMNCs from WT or Nlrp3-KO mice. White blood cells (left) and platelets (right) were counted at intervals (at 0, 3, 7, 14, 21, and 28 days after transplantation). *p < 0.05.