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. 2020 Aug 29;10(9):412. doi: 10.1007/s13205-020-02392-1

Fig. 1.

Fig. 1

Gel showing proteins extracted through different methods. Lane 1: Lysis buffer with 2D-clean-up; Lane 2: lysis buffer with acetone precipitation; Lane 3: phenol–sulfuric extraction with 2D-clean-up; Lane 4: Phenol–sulfuric extraction with acetone precipitation; Lane 5: 10% TCA containing 0.07% β-ME and 1 mM PMSF with acetone washing (0.07% β-ME + 1 mM PMSF); Lane 6: 10% TCA containing 0.07% β-ME and 1 mM PMSF with 2D-clean-up; lane 7: 10% TCA containing 0.07% β-ME with acetone washing (0.07% β-ME); Lane 8: 10% TCA containing 0.07% DTT with acetone washing (0.07% DTT)