Table 2.
Gene | Accession No. | Primers | Sequence (5′–3′) | Product size | Tm |
---|---|---|---|---|---|
P5CS1 | GQ377719.2 | Forward | CTGGTGGTATCTGCCATGTT | 496 bp | 62 °C |
Reverse | TGTATGCGCCCTGTACTTATG | ||||
BADH1 | U12195.1 | Forward | TCCTCTCCTGATGGCTACAT | 858 bp | 62 °C |
Reverse | TGTGAGCAGTTTACCCAGATAC | ||||
Actin-1 | XM_021463392.1 | Forward | CAACTGGGACGATATGGAGAAG | 571 bp | 62 °C |
Reverse | AATGAAGGATGGCTGGAAGAG | ||||
Cu–Zn SOD- | XM_002445626.2 | Forward | CCTCCACGAGTTTGGTGATAC | 296 bp | 62 °C |
Reverse | CCAGTCTTCCACCAGCATTT | ||||
H+-PPase | GQ469975.1 | Forward | CACCTCTCTGGTATCTGGTTTC | 629 bp | 62 °C |
Reverse | GTGCGGGCTCAATTTCTTTC |