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. 2020 Aug 20;2020:1878431. doi: 10.1155/2020/1878431

Figure 2.

Figure 2

Competitive expression between circDDX17 and miR-346. (a) Relative miR-346 expression in prostate cancer tissue samples and corresponding adjacent nontumor tissue samples was determined by qPCR. (b) The miR-346 expression in 22Rv1 and PC-3 cell lines transfected with pcDNA-NC or pcDNA-circDDX17 was determined by qPCR. (c) Relative circDDX17 expression in 22Rv1 and PC-3 cell lines transfected with si-NC, si-circDDX17-1 or si-circDDX17-2 was measured by qPCR. si-circDDX17-1 was used in follow-up experiments. (d) Relative miR-346 expression in 22Rv1 and PC-3 cell lines transfected with si-NC or si-circDDX17 was detected by qPCR. (e) Relative miR-346 expression in 22Rv1 and PC-3 cell lines transfected with pPG-miR-NC, pPG-anti-miR-346, or pPG-miR-346 was detected by qPCR. (f) Relative circDDX17 expression in 22Rv1 and PC-3 cell lines transfected with pPG-miR-NC, pPG-anti-miR-346, or pPG-miR-346 was detected by qPCR. ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001.