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. 2020 Aug 29;17:253. doi: 10.1186/s12974-020-01902-5
Antibody Dilution
GSDMDC1 (Sigma-Aldrich WH0079792M1 clone 3F12-1B2) 1:200
Caspase-1 p10 (Santa Cruz #514) 1:200
Cleaved PARP (Cell Signalling Technology #9541) 1:200
Total Caspase-3 (R&D Systems AF-605-NA) 1:200
Cleaved ROCK-1 (Novus NB100-56596) 1:200
Cleaved caspase-3 (Cell Signalling Technology #9661) 1:200
Cleaved caspase-7 (Cell Signalling Technology #9491) 1:200
Cleaved DFF45 (Cell Signalling Technology #9731) 1:200
Cleaved GSDMD (provided by FS) 1:100
MHC Class II (Santa Cruz Biotechnology sc-59318) 1:200