. 2020 Aug 27;8:e9651. doi: 10.7717/peerj.9651

Table 6. Effects of Allium fistulosum L. extracts on in vitro methane and carbon dioxide emission.

Incubation time (h)	Extract concentration^d, %						SEM^e	p-value^f
	0	1	3	5	7	9		T	L	Q
Methane, mL/g DM
3	3.55^a	3.04^ab	2.75^ab	2.64^ab	2.09^b	2.66^ab	0.32	0.112	0.021	0.126
6	6.80^a	5.11^bc	4.98^bc	4.25^c	5.71^ab	6.25^ab	0.38	0.006	0.892	0.0005
9	11.04	10.85	8.97	10.37	11.03	10.82	0.80	0.468	0.833	0.207
12	12.36^a	9.83^b	7.87^c	8.43^bc	7.84^c	7.94^c	0.50	0.0002	<0.0001	0.002
24	20.06^a	20.78^a	19.23^ab	15.61^bc	15.67^bc	14.04^c	1.20	0.015	0.0003	0.923
Carbon dioxide, mL/g DM
3	57.54^a	58.35^a	47.72^ab	42.73^b	38.87^b	42.12^b	3.46	0.006	0.0004	0.084
6	52.91^a	46.17^ab	49.30^ab	42.84^b	55.12^a	54.34^a	2.94	0.069	0.211	0.048
9	65.96	65.16	61.90	70.29	72.84	70.19	4.28	0.513	0.154	0.901
12	63.97^a	52.48^b	51.13^b	55.00^b	50.89^b	52.48^b	2.87	0.057	0.050	0.104
24	89.69	94.30	94.45	87.70	137.08	86.41	17.15	0.340	0.484	0.533

Notes.

^a–c

Means with different superscript letters in the same row indicate significant differences (P < 0.05).

Allium fistulosum L. extract concentrations are based on quantity of timothy hay (300 mg) substrate.

SEM, standard error of the mean.

T, treatment; L, linear; Q, quadratic effect.

DM, dry matter.

n = 3.