Figure 4.

Increased autofluorescence and IgG depositions at RPE and sub-RPE space of the adult DKO mice. Autofluorescence at 488 nm wavelength (A) and endogenous IgG staining (B) was examined in the retinal sections prepared from WT, DKO 2 and 4 m.o. CXCR5 KO, and NRF2 KO. The representative Western blotting images and quantification results of endogenous IgG heavy (HC) and light chains (LC) from RPE/choroid (C) and the retina (D). The densitometry quantifications for HC and LC in RPE/choroid (E-F) and retina (G-H), were expressed as relative ratios of WB densitometry density between IgG and β-actin (n = 3). P values were denoted: n.s. P > 0.05; *P < 0.05; **P < 0.01; ***P < 0.001.