Reduced zonula occludens-1 (ZO-1) protein levels elevated AMD-associated proteins and microglia marker in the adult DKO. The representative Western blotting images of ZO-1, apolipoprotein E (Apo-E), transmembrane Protein 119 (TMEM119) (A) and β-amyloid (B) in RPE/BM/Choroid protein lysates prepared from WT (6 m.o), DKO (2, 4, 6 m.o.) and CXCR5 KO (6 m.o.) old mice. β-actin used as the loading control. (C-F) The quantification results of the four protein expression levels. The results were expressed as relative ratios of WB densitometry density between target proteins and β-actin (n = 4). P values were denoted: n.s. P > 0.05; **P < 0.01; ***P < 0.001.