Table 2.
Primer sequences and amplification efficiencies (E) of qPCR assays. All sequences are written in the 5′ to 3′ direction. Primers were designed using gene sequences from elephant seal blubber transcriptomes (Deyarmin et al., 2019, Khudyakov et al., 2017)
| Transcript ID | Gene homolog | Protein name | Primer sequence | E (%) |
|---|---|---|---|---|
| TRINITY_DN592797_c2_g1 | PLIN1 | Perilipin 1 | F:GCGGTCAACAAGGACCCAAC R:GGAAACACTCACAGGTGCCG |
94 |
| TRINITY_DN572974_c28_g1 | ADIPOQ | Adiponectin | F: CCAATGTTCCCATTCGCTTTAC R: CATTCCTGGGCTGTACTACTTC |
93 |
| TRINITY_DN581259_c1_g6 | LEP | Leptin | F: ACAGGACCAAAGCCACAGGA R:GCGAGGCCTGAGAAGCACAT |
104 |
| TRINITY_DN585354_c4_g5 | DKK1 | Dickkopf-related protein 1 | F: CCAAGATCTGTAAACCTGTCCTCR: CACAGTAACAGCGCTGGAATA | 103 |
| TRINITY_DN571555_c4_g2 | GPX3 | Glutathione peroxidase 3 | F: CCGGACGGTGTACCCATCAT R: GGCAGGTCTGATTTACTGCCC |
107 |
| TRINITY_DN587426_c4_g1 | CIDEA | Cell death activator CIDE-A | F: CGCGTTGCCAATCTAGACGC R: TCCGTATCCACCACGGTTCC |
100 |
| TRINITY_DN560507_c9_g1 | HMGCS2 | Hydroxymethylglutaryl-CoA synthase 2 | F:TGATGTTCAGTGACTTCCTGTC R: TGTAGGTTTCTTCCAGCGTTAG |
100 |
| TRINITY_DN591927_c4_g2 | CDO1 | Cysteine dioxygenase 1 | F:TGACAAATTCCTGTCCGATAAGT R:TTCTGCCCTCCCTTTCATTAC |
97 |
| TRINITY_DN591034_c2_g1 | TGFBI | Transforming growth factor beta-induced | F:TCCTGAAGGGGGACAATCGC R: GACCCCTTCCCTGTTGAGCA |
97 |
| TRINITY_DN571072_c3_g3 | ACSL1 | Long-chain-fatty-acid CoA ligase 1 | F:GTAGCGATGGTGCTCGGAGA R: ACTTGACACCTGGATGCCCC |
97 |
| TRINITY_DN587150_c2_g2 | AZGP1 | Zinc-alpha-2-glycoprotein 1 | F: TCGTTCTCCCAGTCCTCTATTC R:CCTACCTCAATGACCAGGATTTC |
94 |
| TRINITY_DN551341_c9_g1 | GLRA2 | Glycine receptor subunit alpha-2 | F: GTCTCCAGACAACACAAGGAG R: CCCATCCCATAACCACTGAAA |
107 |
| TRINITY_DN587322_c7_g1 | PIAS4 | E3-SUMO-protein ligase PIAS4 | F:GCGGACTTAAACACGAACTTGTCA R: GAGCTCTTCTTGGCGTAGCG |
106 |
| TRINITY_DN592891_c7_g1 | MGST1 | Microsomal glutathione S-transferase 1 | F: TTAACGACTGAGCCACCCAGG R:GCATCAAGATGAACCACAAGTTGG |
100 |
| TR6987 c5_g2 (from Khudyakov et al., 2017) | NONO | Non-POU domain-containing octamer-binding protein | F:GAGGAAGGTTTCGGACTGTAAG R:GCGGAGATTGCCAAAGTAGA |
95 |
| TR33370 c1_g1 (from Khudyakov et al., 2017) | YWHAZ | 14-3-3 protein zeta/delta | F: AGCAGAGAGCAAAGTCTTCTATT R: GACTGATCCACAATCCCTTTCT |
100 |