Table 1.

Summary of PTM identified in deglycosylated peptides from HCoV-19 S protein and hACE2.

Sites	PTM	Sequences	Occupancy (%)
HCoV-19 S protein
61, 74	N-glycosylation	SSVLHSTQDLFLPFFSNaVTWFHAIHVSGTNaGTK	100
74	N-glycosylation	HAIHVSGTNaGTKRF	100
78	Methylation	RaFDNPVLPFNDGVYFASTEK	100
122	N-glycosylation	TQSLLIVNNaATNVVIK IVNNaATNVVIKVCEF	100 100
165	N-glycosylation	VYSSANNaCTFEYVSQPFLMDLEGK	100
224	Methylation	DLPQGFSALEaPLVDLPIGINITR	100
234	N-glycosylation	VDLPIGINaITRF	100
282	N-glycosylation	YNENaGTITDAVDCALDPLSETK NENaGTITDAVDCALDPLSETKCTL	100 100
331	N-glycosylation	RVQPTESIVRFPNaITNL	100
331, 334	N-glycosylation	FPNaITNLCPFGEVFNaATR	100
340	Methylation	FPNITNLCPFGEaVFNATR	2.02
603	N-glycosylation	GGVSVITPGTNaTSNQVAVL	43.50
616	N-glycosylation	YQDVNaCTEVPVAIHADQL	100
654, 661	Methylation	AGCLIGAEaHVNNSYEaCDIPIGAGICASYQTQTNSPR	100
657	N-glycosylation	AGCLIGAEHVNaNSYECDIPIGAGICASYQTQTNSPR IGAEHVNaNSYECDIPIGAGICASY	74.80 79.76
709, 717	N-glycosylation	SNaNSIAIPTNaF	100
801	N-glycosylation	TPPIKDFGGFNaFSQILPDPSKPSK NaFSQILPDPSKPSKRSF	100 100
1074	N-glycosylation	NaFTTAPAICHDGK	100
1098	N-glycosylation	VSNaGTHWF	100
1134	N-glycosylation	VSGNCDVVIGIVNaNTVY	100
1158, 1173	N-glycosylation	NaHTSPDVDLGDISGINaASVVNIQKEIDRLNEVAK	99.17
1194	N-glycosylation	NLNaESLIDLQELGK	99.73
hACE2
53	N-glycosylation	FNHEAEDLFYQSSLASWNYNTNaITEENVQNMNNAGDK NYNTNaITEENVQNMNNAGDKW	100 100
68	Methylation	NYNTNITEENVQNMNNAGDKaW	100
84	Hydroxyproline	EQSTLAQMYPaLQEIQNLTVK	20.74
90	N-glycosylation	WSAFLKEQSTLAQMYPLQEIQNaLTVK	100
103	N-glycosylation	LQLQALQQNaGSSVLSEDKSK QLQALQQNaGSSVL	100 100
253	Hydroxyproline	LMNAYPaSYISPIGCLPAHLLGDMWGR	98.35
263	Hydroxyproline	LMNAYPSYISPIGCLPaAHLLGDMWGR	88.57
284	Hydroxyproline	FWTNLYSLTVPaFGQKPNIDVTDAMVDQAWDAQR	4.06
289	Hydroxyproline	PaNIDVTDAMVDQAWDAQR	1.70
321	Hydroxyproline	FFVSVGLPaNMTQGFWENSMLTDPGNVQK	58.24
322	N-glycosylation	FFVSVGLPNaMTQGFWENSMLTDPGNVQK VSVGLPNaMTQGF	100 100
329	Methylation	FFVSVGLPNMTQGFWEaNSMLTDPGNVQK	100
346	Hydroxyproline	AVCHPaTAWDLGK	88.88
415	Hydroxyproline	NGANEGFHEAVGEIMSLSAATPaK	0.68
432	N-glycosylation	SIGLLSPDFQEDNaETEINFLLK LSPDFQEDNaETEINF	100 100
451	Hydroxyproline	QALTIVGTLPaFTYMLEK	43.59
469	Hydroxyproline	GEIPaKDQWMK	4.09
546	N-glycosylation	CDISNaSTEAGQK HKCDISNaSTEAGQKLF	100 100
565	Hydroxyproline	SEPaWTLALENVVGAK	0.37
583	Hydroxyproline	NMNVRPaLLNYFEPLFTWLK	2.12
612	Hydroxyproline	NSFVGWSTDWSPaYADQSIK	0.21
690	N-glycosylation	ISFNFFVTAPKNaVSDIIPR VTAPKNaVSDIIPRTEVEKAIRM NaVSDIIPRTEVEK	100 100 100

^aModified sites.