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. 2020 Aug 25;11(34):3244–3255. doi: 10.18632/oncotarget.27696

Figure 1. Integrated proteomics and N-glycoproteome analysis workflow applied to urine samples from bladder cancer patients and healthy subjects.

Figure 1

Following filtration with 3kDA filters, urinary proteins were concentrated and digested with trypsin. Each sample was labeled with 10-plex Tandem Mass Tags (TMT) labeling kit and pooled. One-tenth of the pooled sample was used for global proteomics experiment and remaining was enriched for N-glycoproteomic analysis. Samples were analyzed in Q Exactive HF-X Hybrid Quadrupole-Orbitrap mass spectrometer and the files were searched against Mascot and Sequest HT search engines. Data analysis was carried out using Perseus. exe 1.6.5.0.