Table 1.
Significant changes in predicted drug-resistance-related orthologs using KEGG after anti-Helicobacter pylori treatment in stool samples
| Drug-resistance-related orthologs | Alteration trend after treatment | Mean relative frequency before treatment | Mean relative frequency after treatment | Fold change* | Q value† |
| Successful eradication group | |||||
| K08164 MFS transporter, DHA1 family, chloramphenicol-resistance protein | ↑ | 4.85E-07 | 1.11E-06 | 2.28 | 0.024 |
| K08221 MFS transporter, aromatic compound/drug exporter family, multidrug-resistance protein | ↑ | 4.79E-07 | 1.08E-06 | 2.26 | 0.026 |
| K03327 multidrug-resistance protein, multidrug and toxin extrusion family | ↓ | 9.64E-04 | 8.26E-04 | 0.86 | 0.011 |
| K03543 multidrug-resistance protein A | ↓ | 2.06E-04 | 1.57E-04 | 0.76 | 0.008 |
| K08169 MFS transporter, DHA2 family, multidrug-resistance protein | ↓ | 1.76E-04 | 1.30E-04 | 0.74 | 0.024 |
| K08223 MFS transporter, fosmidomycin resistance family, fosmidomycin-resistance protein | ↓ | 2.53E-04 | 1.84E-04 | 0.73 | 0.007 |
| Failed treatment group | |||||
| K08164 MFS transporter, DHA1 family, chloramphenicol-resistance protein | ↑ | 4.27E-07 | 1.21E-06 | 2.82 | 0.005 |
| K08221 MFS transporter, aromatic compound/drug exporter family, multidrug-resistance protein | ↑ | 4.01E-07 | 1.17E-06 | 2.92 | 0.005 |
| K02546 methicillin-resistance regulatory protein | ↑ | 1.12E-08 | 4.10E-08 | 3.64 | 0.007 |
| K07681 two-component system, NarL family, vancomycin-resistance sensor histidine kinase VraS(EC:2.7.13.3) | ↑ | 7.10E-07 | 2.56E-06 | 3.61 | 0.007 |
| K08151 MFS transporter, DHA1 family, tetracycline-resistance protein | ↑ | 2.00E-06 | 6.58E-06 | 3.29 | 0.009 |
| K08152 MFS transporter, DHA1 family, multidrug-resistance protein B | ↑ | 9.71E-06 | 1.44E-05 | 1.48 | 0.025 |
| K08153 MFS transporter, DHA1 family, multidrug-resistance protein | ↑ | 3.52E-06 | 7.81E-06 | 2.22 | 0.009 |
| K08161 MFS transporter, DHA1 family, multidrug-resistance protein | ↑ | 3.01E-05 | 5.75E-05 | 1.91 | 0.017 |
| K08170 MFS transporter, DHA2 family, multidrug-resistance protein | ↑ | 3.97E-06 | 1.29E-05 | 3.25 | 0.007 |
| K11814 multidrug-resistance protein EbrA | ↑ | 1.70E-09 | 1.90E-08 | 10.61 | 0.005 |
| K11815 multidrug-resistance protein EbrB | ↑ | 6.52E-09 | 2.77E-08 | 4.21 | 0.020 |
*The fold change was calculated as the ratio of the mean relative frequency after treatment to the mean relative frequency before treatment.
†Paired t-test after arcsine transformation of relative frequency and q values were used after adjustment for multiple comparison by the FDR.
DHA, drug:H+ antiporter; FDR, false discovery rate; KEGG, Kyoto Encyclopedia of Genes and Genomes; MFS, major facilitator superfamily.