Fig. 5.

Thermostability of purified CD81. Effect of temperature on secondary structure measured by CD spectroscopy over a range of different temperatures for A) CD81-SMALP and B) CD81-DDM. Temperatures ranged from 25 °C to 90 °Cin 5 °C increments. Eighteen technical replicates were taken at each condition, n = 2. C) The temperature dependenceof the CD signal at 207 nm for both CD81-SMALP (black closed circles) and CD81-DDM (grey open circles). D & E) Effect of temperature on protein aggregation analysed by Western blot using an anti-CD81 antibody (mAb 2s131) for D) CD81-SMALP and E) CD81-DDM. F) Effect of increasing temperature on the binding of CD81 to a conformation sensitive anti-CD81 (mAb 1s337) antibody, using an ELISA. Black solid circles are CD81-SMALP, open grey squares are CD81-DDM. Data are mean ± SEM, n = 3.