Figure 2.

Infection of murine EMOs with C. trachomatis. (A) Cartoon diagram depicting the method of infecting EMOs with C. trachomatis by mechanical fragmentation. (B) Confocal micrographs of an EMO infected with mCherry-expressing C. trachomatis (red, left panel, mCherry). Infected EMOs were fixed at 32 hpi and immunostained with anti-C. trachomatis MOMP (green, middle panel, MOMP). The merge, including a brightfield image, is shown on the right. Higher magnification of the indicated boxed area is shown in the inset of each panel. A single plane across the middle of the inclusion is shown. Scale bar: 40 μm. (C) Confocal micrographs of an EMO infected with mCherry-expressing C. trachomatis (red, middle panels, mCherry) fixed at 32 hpi and stained with SYTOX^TM Green (green, left panels, DNA) and Phalloidin (red, middle panels, F-Actin). The merge is shown on the right. A single plane across the middle of the EMO and inclusions is shown. Top panels: whole EMO. Scale bar: 40 μm. Bottom panels: higher magnification of indicated boxed area. Inclusions are indicated by arrow heads. Scale bar: 20 μm.