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. 2020 Aug 14;11:538. doi: 10.3389/fendo.2020.00538

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Copyright © 2020 Tao, Ji, Huang, Fan, Liu, Liu and Tao.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Nucleotide and deduced amino acid sequences and phylogenetic tree of caMC4R. (A) Nucleotide and deduced amino acid sequences of caMC4R. Positions of nucleotide and amino acid sequences are indicated on both sides. Shaded boxes denote putative TMD1-7. Potential phosphorylation sites are present in oval frame. Underline show PMY, DRY, DPxxY motifs. Open boxes denote the consensus sequence for N-linked glycosylation sites. Asterisk (*) indicates stop codon. (B) Phylogenetic tree of MC4R proteins. The tree was constructed by the neighbor-joining (NJ) method. Numbers at nodes indicate the bootstrap value, as percentages, obtained for 1,000 replicates. Black dot shows caMC4R. MC4Rs: Culter alburnus (topmouth culter, MT163518), Scatophagus argus (spotted scat, KU760724.1), Dicentrarchus labrax (European sea bass, CBN82190.1), Lepisosteus oculatus (spotted gar, XP_006634516.1), Danio rerio (zebrafish, NP_775385.1), Vulpes lagopus (arctic fox, ACN55093.1), Ctenopharyngodon idella (grass carp, AOZ60534.1), Paralichthys olivaceus (Japanese flounder, ADP09415.1), Squaliobarbus curriculus (barbel chub, ADV40875.1), Takifugu rubripes (fugu, NP_001027732.1), Mylopharyngodon piceus (black carp, ADV40871.1), Tyto alba (barn owl, ATN96237.1), Columba livia (rock dove, XP_021153678.1), Bos taurus (cattle, NP_776535.1), Alligator sinensis (Chinese alligator, XP_006025279.1), Anser anser (greylag goose, ABF19809.1), Pongo abelii (Sumatran orangutan, XP_002828309.1), Equus caballus (horse, XP_001489706.1), Felis catus (cat, XP_019670932.2), Gallus gallus (chicken, AEP17334.10), Sus scrofa (pig, ABD28176.1), Mus musculus (mouse, NP_058673.2), Capra hircus (goat, NP_001272520.1), and Homo sapiens (human, NP_005903.2).