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. 2020 Aug 14;11:538. doi: 10.3389/fendo.2020.00538

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Copyright © 2020 Tao, Ji, Huang, Fan, Liu, Liu and Tao.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Nucleotide and deduced amino acid sequences and phylogenetic tree of caMRAP2a and caMRAP2b. (A) Nucleotide and deduced amino acid sequences of caMRAP2a. (B) Nucleotide and deduced amino acid sequences of caMRAP2b. The positions of nucleotide and amino acid sequences are indicated on both sides. Shaded box shows putative TMD. Open boxes denote putative N-linked glycosylation sites. Underline show initiation and stop codons. Asterisk (*) indicates stop codon. (C) Phylogenetic tree of MRAP2s. The tree was constructed by the NJ method. Numbers at nodes indicate the bootstrap value, as percentages, obtained for 1,000 replicates. Black triangle dot show caMRAP2a and caMRAP2b. MRAP2s: Culter alburnus (topmouth culter, MRAP2a: MT163516, and MRAP2b: MT163517), Danio rerio (zebrafish, MRAP2a: F8W4H9.1, and MRAP2b: F8W4H9.1), Epinephelus coioides (orange-spotted grouper, QED39647.1), Oryzias latipes (Japanese medaka, XP_023809099.1), Maylandia zebra (zebra mbuna, XP_004568825.1), Amphiprion ocellaris (anemonefish, XP_023122806.1), Kryptolebias marmoratus (mangrove rivulus, XP_017267334.1), Oncorhynchus mykiss (rainbow trout, XP_021467183.1), Oreochromis niloticus (Nile tilapia, XP_003458293.2), Gallus gallus (chicken, ALO81626.1), Ictidomys tridecemlineatus (thirteen-lined ground squirrel, XP_021581743.1), Oncorhynchus tshawytscha (Chinook salmon, XP_024278413.1), Mus caroli (Ryukyu mouse, XP_021029091.1), Pteropus alecto (black flying fox, XP_006926405.1), Puma concolor (puma, XP_025781535.1), Homo sapiens (human, AAH10003.2), Bubalus bubalis (water buffalo, XP_006054803.2), Macaca nemestrina (pig-tailed macaque, XP_011764298.1), Triplophysa tibetana (MRAP2a: KAA0703529.1 and MRAP2b: KAA0720858.1), Anabarilius grahami (Kanglang fish, MRAP2b ROJ29330.1), Carassius auratus (goldfish, MRAP2a: XP_026139519).