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. 2020 Aug 14;11:538. doi: 10.3389/fendo.2020.00538

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Copyright © 2020 Tao, Ji, Huang, Fan, Liu, Liu and Tao.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Constitutive activities of caMC4R in cAMP and ERK1/2 pathways. HEK293T cells were transfected with increasing concentrations of caMC4R plasmids. Cells transfected with empty pcDNA3.1 vector was considered as control group. (A) cAMP levels were were measured by RIA. The curve was made with data from three independent experiments. (B,C) The ERK1/2 phosphorylation levels were detected by western blot. Values are expressed as mean ± SEM (n = 3). Asterisk (*) showed significant difference of cAMP and pERK1/2 levels compared with control group (P < 0.05) (One-way ANOVA followed by Tukey-test).