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. 2020 Aug 14;8:778. doi: 10.3389/fcell.2020.00778

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Copyright © 2020 Santos, Silva, Fortuna, Silveira, Orge, de Santana, Sampaio, Paredes, Ribeiro-dos-Santos and Soares.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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In vitro endothelial cell migration. (A) Representative image of the migration of HUVEC cells, stained with violet crystal, after 9 or 18 h of incubation in conditioned medium of MSC (CM-MSC) or MSC_LIF (CM-MSC_LIF). (B) Quantification of the percentage of variation in the wound area (% migration area). The two-way ANOVA test and the Bonferroni post-test were used to analyze the differences among the groups. Values are expressed as mean ± SEM of at least three independent experiments. ***p < 0.001. Scale bars = 250 μm.