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. 2020 Aug 14;10:1433. doi: 10.3389/fonc.2020.01433

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Copyright © 2020 Han, Wu and Cui.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Expression pattern of LINC00337 in cervical cancer and phenotypic characterization of HeLa-SFCs. (A) The expression pattern of LINC00337 in cervical cancer samples (n = 306) and adjacent normal samples (n = 13) from TCGA database. (B) LINC00337 expression was quantified in NCECs and cervical cancer cell lines HeLa, SiHa, CaSki, and C33A using the qRT-PCR analysis, normalized to GAPDH expression; data are shown as mean ± standard deviation of three technical replicates and analyzed by one-way ANOVA (*p < 0.05 vs. NCECs). (C) Identification of CD44⁺/CD24^low/−SFCs by fluorescence-activated cell sorting analysis; (D) Multi-drug resistance of CD44⁺/CD24^low/−SFCs; CD44⁺/CD24^low/−SFCs were maintained in sphere culture medium separately supplemented with working concentrations of cisplatin, doxorubicin and epirubicin, compared with parental HeLa cells cultured in sphere culture medium without these drugs (×40). (E) The mRNA expression of Nanog, Sox2, and Oct4 in CD44⁺/CD24^low/−SFCs and non-CD44⁺/CD24^low/−SFCs were determined by the qRT-PCR analysis, normalized to GAPDH expression. (F) Representative Western blots of Nanog, Sox2 and Oct4 in CD44⁺/CD24^low/−SFCs and non-CD44⁺/CD24^low/−SFCs and their quantitative analyses. (G) LINC00337 expression was quantified in CD44⁺/CD24^low/−SFCs and non-CD44⁺/CD24^low/−SFCs using the qRT-PCR analysis, normalized to GAPDH expression; data are shown as mean ± standard deviation of three technical replicates and analyzed by non-paired t-test (*p < 0.05 vs. non-CD44⁺/CD24lo^w/−SFCs).