FIGURE 1.

Potential differential spatial distribution and clustering behavior of RNA transcripts in cells, which share the same identity based on single-cell RNA sequencing. (A) Single-cell RNA-seq workflow, which typically yields t-SNE plots shown in (B). B Cells that are classified as belonging to the same subtype/group based on RNA transcript count might differ in the subcellular localization of various RNA transcripts. (C) Spatially confined RNA transcripts may exhibit non-clustered spatial distribution or localize in clusters. (D) Spatial subcellular localization of RNA transcripts may be correlated with or independent of specific cellular structures, organelles or markers.