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. 2020 Aug 14;10:1424. doi: 10.3389/fonc.2020.01424

Figure 6.

Figure 6

The proliferation inhibition, apoptotic induction, and antimigration effect of KPT-185 on PC9 and PC9GR cells. (A) The representative images of colony formation by PC9 and PC9GR cells after being treated with KPT-185 at 0, 0.125, 0.25, 0.5 μM for 9 days. The inhibition is dose dependent and similar between PC9 and PC9GR cells. (B) Quadrant graphs of cell apoptosis analysis for PC9 and PC9GR after being treated with KPT-185 at five concentrations for 48 h using flow cytometry. X-axis represents Annexin V-FITC, and y-axis represents PI. Q1, Q2, Q3, and Q4, respectively, represent dead cell fragments, late apoptosis, early apoptosis, and normal cell community. (C) The ratios of apoptotic cells at different concentrations in PC9 cells. (D) The ratios of apoptotic cells at different concentrations in PC9GR cells. (E) The representative images of migration inhibition after KPT-185 treatments in PC9 and PC9GR cells by wound-healing assay (magnification at 100X). (F) The bar plot of migration inhibition rates at 0, 0.25, 0.5, 1, and 2 uM in PC9 cells. (G) The bar plot of migration inhibition rates at 0, 0.25, 0.5, 1, and 2 uM in PC9GR cells. The bar height represents mean ± SEM of three independent experiments (n = 3, **P < 0.01, ***P < 0.001 compared to controls).