Table 1.
Characteristics of amplicons and primer sequences of TLR2, TLR4, TLR9, CD14, IL23, IL23R, and IL1R genes.
| Frag. No. | Gene | OMIM No. | Chromosome coordinates (GRCh37/hg19) | Primer sequence (5′→3′) | Amplicon length (bp) |
|---|---|---|---|---|---|
| 1. | TLR2 | 603028 | chr4:154620045+154627075 | F:CTGGTCTTTCTATAGGGTTAGGCTTT | 7031 |
| R:TCTCTTAGCAGGAAGAAAGAATGACA | |||||
| 2. | TLR4 | 603030 | chr9:120466961+120479323 | F:ACTCAAGAAGCCACAGAGATCAAATA | 12363 |
| R:ACTAGTACATGAGACATGGAAACACA | |||||
| 3. | TLR9 | 605474 | chr3:52254989-52260328 | F:CTAGATCAGAAGGAGAGTGGGAAGAA | 5340 |
| R:CTGTCCCGTCTTTATGGCAATTTTC | |||||
| 4. | CD14 | 186940 | chr5:140011176-140015540 | F:AAAGTTCCCAGCCAAAAGCTATATTC | 4365 |
| R:TTCTTTTCTTGAGGAGGACAGATAGG | |||||
| 5. | IL23R (part 1) | 607562 | chr1:67627253+67635706 | F:CAAACTACGTACTCCCTTGCATATTT | 8454 |
| R:AGAGCAACATTTTAAATCTAACGGGG | |||||
| 6. | IL23R (part 2) | chr1:67647462+67649273 | F:CTTTGATGATGGTGATGTACAGATGG | 1812 | |
| R:AGAAGAAGAATAGAGCAGGGATTTAAGCA | |||||
| 7. | IL23R (part 3) | chr1:67665995+67673339 | F:GATTACAAGCGTGAGCCATAAATTCT | 7345 | |
| R:CAAAAGGCTCTTCTTACTTAGTGGTG | |||||
| 8. | IL23R (part 4) | chr1:67684715+67685915 | F:CACATTTGGACTTTCTGGAGATTTGA | 1201 | |
| R:CATGCCCAGCTAATTTTCGTATTTTT | |||||
| 9. | IL23R (part 5) | chr1:67702124+67706145 | F:CATGAAATTAGTGGACACACCTTGA | 4022 | |
| R:GAAAGACATTTGTAGAGAGTTTGGCA | |||||
| 10. | IL23R (part 6) | chr1:67720545+67724949 | F:GGGAAAAAGTGGGATGTTTATGACTT | 4405 | |
| R:TGAAAACATGTGTCCATGTGAAGATT | |||||
| 11. | IL23 | 605580 | chr12:56728218+56734024 | F:AAACTCGCTGGCATTTCCTATTATTT | 5807 |
| R:TAATTTTCAACATATGCAGGTCCCAC | |||||
| 12. | IL1R | 147810 | chr2:102770366+102783183 | F:ATTCACACTTCACTCATGTGTTCTTC | 12818 |
| R:AATGAACAGGAGGTAGATTCTGGAAA |
F, forward; R, reverse; chr, chromosome; OMIM, Online Mendelian Inheritance in Man; frag., fragment.