FIGURE 2.
PmCQ2Δ0442 characteristics. (A) Bacterial growth curves based on OD600 nm reading. (B) Colony morphology. (C) PmCQ2Δ0442 and PmCQ2 strains after standing for the same time. (D–F) Capsule thickness of fresh cultured PmCQ2 (D), PmCQ2Δ0442 (E) and PmCQ2Δ0442/pPm0442 (F) by TEM. (G) The content of capsule polysaccharide in PmCQ2, PmCQ2Δ0442, and PmCQ2Δ0442/pPm0442. (H) The number of PmCQ2Δ0442 associated with macrophages. (A,B) were pooled from three independent experiments with three replicates per group. Panels (G,H) were pooled from two independent experiments with eight replicates per group. And (H) were determined by multiple comparative analysis, (G) was analyzed by unpaired t-test. All data were expressed as means ± SD. (**P < 0.01, ***P < 0.001, ****p < 0.0001).