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. 2020 Aug 14;11:1972. doi: 10.3389/fmicb.2020.01972

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Copyright © 2020 He, Qin, Xu, Li, Wu, Duan, Du, Fang, Hardwidge, Li and Peng.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Virulence gene transcription in vivo. (A) Heat map for clustering of DEGs (FC ≥ 1.5) of PmCQ2 and PmCQ2-Δ0442 in vivo. (B) The up/down-regulated DEGs (FC ≥ 1.5) of PmCQ2 and PmCQ2-Δ0442 in vivo. (C) TonB receptor-related DEGs (FC ≥ 1.5) in RNA-seq and in RT-PCR (n = 3). (D) Iron-related DEGs (FC ≥ 1.5) in RNA-seq and in RT-PCR (n = 3). (E) Capsule synthesis and transport- related DEGs (FC ≥ 1.5) in RNA-seq and in RT-PCR (n = 3). Panels (C–E) were representative of two independent experiments with 3 replicates per group and analyzed by multiple comparative analysis, and expressed as means ± SD (*p < 0.05, **p < 0.01, ***p < 0.001).