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. 2020 Aug 14;11:1234. doi: 10.3389/fpls.2020.01234

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Copyright © 2020 Salonia, Ciacciulli, Poles, Pappalardo, La Malfa and Licciardello

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Different approaches for Agrobacterium-mediated transformation of citrus varieties starting from different tissue sources. (A) Juvenile internodal segments from nucellar seedlings. (B) Embryogenic callus obtained from unfertilized ovules. (C) Mature tissues, such as internodal segments from flushes and leaves. All the shoots or embryos, obtained from regeneration of transformed explants, are grafted onto vigorous rootstocks in order to induce early flowering, with the final aim to shorten the evaluation of the modified traits.