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. 2020 Aug 14;11:1912. doi: 10.3389/fmicb.2020.01912

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Copyright © 2020 Zhang, Goraya, Chen, Xu, Hong, Zhu and Chen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Identification of differentially expressed genes in response to influenza A/WSN/33 virus infection. (A) Heat map showing microarray analysis of gene expression profile in A549 cells infected with mock and influenza A/WSN/33 virus for 12 h (MOI = 1). The identified genes were differentially expressed by ≥ 2-fold (n = 3) between mock and virus infection. Samples were obtained from three independent experiments. (B) The expression of the selected genes from microarray analysis was confirmed by RT-PCR. (C) The expression level of ZC3HAV1 in influenza A/WSN/33 virus-infected A549 cells was measured by Western blotting. (D) The expression of ZC3HAV1 in influenza A/WSN/33 virus-infected BALB/c mice was measured by qRT-PCR. Data are represented as mean ± SD. ^∗p < 0.05; ^∗∗p < 0.01.