Skip to main content
. 2020 Aug 14;11:1912. doi: 10.3389/fmicb.2020.01912

FIGURE 3.

FIGURE 3

Knockdown of endogenous ZC3HAV1 expression significantly increases the replication of IAV in A549 cells. A549 cells stably expressing specific sh-RNAs targeting ZC3HAV1 or luciferase control were infected with or without WSN virus at an MOI of 1.0, and the extracts were harvested at the indicated time points after infection. (A) qRT-PCR was performed to measure the interference efficiency of the sh-ZC3HAV1. Data are represented as mean ± SD. ∗∗p < 0.01. (B) Protein extracts collected from the A549 cells expressing specific sh-ZC3HAV1 infected with WSN virus at the indicated time points (0 and 12 hpi) were subjected to Western blotting analysis with antibodies specific to ZC3HAV1 and WSN-NP. (C,D) qRT-PCR was performed to measure the expression of ZC3HAV1 after treatment with IFN-β and poly(I:C) in ZC3HAV1 knockdown A549 cells. Data are represented as mean ± SD. ∗p < 0.05; ∗∗p < 0.01. (E) A549 cells expressing specific sh-ZC3HAV1 were infected with WSN virus, and qRT-PCR was performed to detect the mRNA expression of WSN-NP. Data are represented as mean ± SD. ∗∗p < 0.01. (F) The cell culture supernatants derived from sh-Luc and sh-ZC3HAV1-expressing A549 cells were harvested to determine the viral titers by plaque assay using MDCK cells. Data are represented as mean ± SD. ∗∗p < 0.01.