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. 2020 Aug 14;11:1912. doi: 10.3389/fmicb.2020.01912

FIGURE 4.

FIGURE 4

ZC3HAV1 knockdown has significant effect on IRF3 signaling and some cytokine expression in IAV-infected cells. (A) Bar graph shows the relative abundance of p-IRF3 protein (normalized to that of IRF3) from three experiments by using ImageJ software in A549 cells expressing sh-ZC3HAV1 or sh-Luc in response to WSN infection. (B) qRT-PCR was performed to detect the mRNA expression of IFN-β in A549 cells expressing sh-ZC3HAV1 or sh-Luc in response to WSN infection. Data are represented as mean ± SD. ∗p < 0.05; ∗∗p < 0.01. (C) The expression of IFN-β mRNA was significantly reduced after poly(I:C) stimulation in sh-ZC3HAV1-expressedA549 cells, which was assessed by qRT-PCR. Data are represented as mean ± SD. ∗p < 0.05. (D) qRT-PCR was performed to detect the expression of MxA mRNA in A549 cells expressing sh-ZC3HAV1 or sh-Luc in response to WSN infection. Data are represented as mean ± SD. ∗∗p < 0.01. (E,F) qRT-PCR analysis determined that the expression of TNF and IL-6 mRNAs was suppressed in A549 cells expressing sh-ZC3HAV1 in comparison to the cells expressing sh-Luc after the IAV infection. Data are represented as mean ± SD. ∗p < 0.05; ∗∗p < 0.01.