FIGURE 7.

Expression of ZC3HAV1 is induced by the infection of Sev and inhibits the virus replication. (A) qRT-PCR was performed to detect the mRNA expression of Sev-NP and ZC3HAV1 in the virus-infected A549 cells at 0, 3, 6, 9, 12, and 24 hpi. (B) The expression level of ZC3HAV1 in Sev-infected A549 cells was measured by Western blotting. (C,D) A549 cells expressing specific sh-ZC3HAV1 were infected with Sev. RT-PCR and qRT-PCR were then performed to detect the mRNA expression of ZC3HAV1 and Sev-NP. Data are represented as mean ± SD. ^∗∗p < 0.01. (E,F) A549 cells overexpressing ZC3HAV1 were infected with Sev, and RT-PCR and qRT-PCR were performed to detect the mRNA expression of ZC3HAV1 and Sev-NP. Data are represented as mean ± SD. ^∗∗p < 0.01.