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. 2020 Aug 14;8:945. doi: 10.3389/fbioe.2020.00945

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Copyright © 2020 Arnesen, Kildegaard, Cernuda Pastor, Jayachandran, Kristensen and Borodina.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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β-farnesene production by the sesquiterpene platform strain. The cumulative effect of engineering strategies for β-farnesene production. AaBFS, A. annua β-farnesene synthase; HMG, HMG-reductase; ERG12, mevalonate kinase; IDI, isopentyl diphosphate isomerase; ERG20, farnesyl diphosphate synthase; ACL, ATP citrate lyase 1; SeACS, S. enterica acetyl-CoA synthetase; pERG11_SQS, substitution of the native squalene synthase promoter with the pERG11-promoter. Standard deviations from the values of three biological replicates are represented by error bars. Statistical significance (students t-test, two-tailed, p < 0.05) is denoted by the letters a, b, c, and d.