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. 2020 Aug 14;8:945. doi: 10.3389/fbioe.2020.00945

FIGURE 3.

FIGURE 3

Limonene and valencene production by platform strains. (A) Limonene production in base and monoterpene platform strain. ClLS, C. lemon limonene synthase; PfLS, P. frutescens limonene synthase. The monoterpene platform strain contains the following gene overexpressions of HMG, ERG12, ACL, SeACS, IDI, ERG20F88W–N119W, and swapping the native squalene promoter (pERG11_SQS). (B) Valencene production in base and sesquiterpene platform strain. CnVS, C. nootkatensis valencene synthase. The sesquiterpene platform strain contains the same modifications as the monoterpene platform strain, with the exception of ERG20 instead of ERG20F88W–N119W. Standard deviations from the values of three biological replicates are represented by error bars. Double asterisks marks significant change (student’s t-test, two-tailed, p < 0.001).