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. 2020 Aug 14;8:945. doi: 10.3389/fbioe.2020.00945

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Copyright © 2020 Arnesen, Kildegaard, Cernuda Pastor, Jayachandran, Kristensen and Borodina.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Squalene and 2,3-oxidosqualene production by the triterpene platform strain. HMG, HMG-reductase; ERG12, mevalonate kinase; pERG7_100bp and pERG7_50bp, truncation of the native lanosterol synthase promoter to 100 or 50 bp, respectively, immediately upstream of the ERG7-gene; ACL, ATP citrate lyase 1; SeACS, S. enterica acetyl-CoA synthetase; IDI, isopentyl diphosphate isomerase; ERG20, farnesyl diphosphate synthase; SQS, squalene synthase; SQE, squalene epoxidase; DGA1, diacylglycerol O-acyltransferase 1. Standard deviations from the values of three biological replicates are represented by error bars. Asterisk marks statistical significant changes compared to ST6512 (student’s t-test, two-tailed, p < 0.05).