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. 2020 Aug 14;8:945. doi: 10.3389/fbioe.2020.00945

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Copyright © 2020 Arnesen, Kildegaard, Cernuda Pastor, Jayachandran, Kristensen and Borodina.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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β-carotene production by the diterpene/carotenoid platform strains. XdCrtI, X. dendrorhous phytoene desaturase; XdCrtYB, X. dendrorhous bi-functional phytoene synthase/lycopene cyclase; ERG20^F88C, mutated version with geranylgeranyl diphosphate synthase activity; GGPPS, geranylgeranyl diphosphate synthase. The diterpene/carotenoid (C20/C40) platform strain contains the following gene overexpressions HMG, ERG12, ACL, SeACS, and IDI, while the native squalene promoter was swapped (pERG11_SQS). Average values and standard deviations for each strain are based on two individual clones with three biological replicates each. Statistical non-significance is marked with ns (student’s t-test, two-tailed, p > 0.05).