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. 2020 Aug 14;7:206. doi: 10.3389/fmolb.2020.00206

FIGURE 5.

FIGURE 5

Effect of low pH and simulated gastric fluid on fibril structure and seeding ability. TEM images of HEWL6.3 (A) and HLZ6.3 (B) after incubation for 24 h at pH 2, 65°C, while shaking at 300 rpm. (C) MALDI-TOF spectra for native HLZ and various fibrils. Effect of simulated gastric fluid on HEWL fibril structure (D), and on the ability of HEWL6.3S and HEWL2S to seed HLZ fibrillation (E). Native human lysozyme was incubated with HEWL fibrils in simulated gastric fluid, pH 1.2, 37°C, containing pepsin, over nearly 4 days. Different HEWL fibril seeds were used: HEWL6.3 pre-treated with pepsin (black curves), and HEWL6.3 (red curves) and HEWL2 (cyan curves) sonicated fibrils (n = 12 for each seed type).