FIGURE 3.

Silencing of ANXA7 upregulates COX2 and PGE₂ in HESCs. HESCs were transfected by non-targeting (NT) or siRNA targeting ANXA7 and then decidualized for 6 days with 0.5 μM 8-Br-cAMP and 1 μM MPA (CM). (A) Arithmetic means ± SEM (n = 8) of COX2 mRNA levels. (B) Original Western blot (left) and arithmetic means ± SEM (n = 5, right) of COX2 protein abundance in HESCs. (C) Arithmetic means ± SEM (n = 14) of PGE₂ levels measured by ELISA. ANOVA or Student’s t-test was used to calculate statistical significance when appropriate. *P < 0.05, **P < 0.01, ***P < 0.001.