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. 2020 Aug 14;7:413. doi: 10.3389/fmed.2020.00413

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Copyright © 2020 Zhou, Cui, Liu, Yue, Xie, Ding, Xu, Han and Zhang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) Immunofluorescence staining (DAPI) on 20-μm slides from fresh frozen NP tissue (CRSwNPs) and control inferior turbinate mucosa (healthy control) performed with polyclonal antibodies against rabbit BRD4. (B) The relative mRNA expression of BRD4 in tissue homogenates from patients with CRSwNP (n = 24) and controls (n = 24) after qRT-PCR analysis normalized to the housekeeping gene GAPDH. (C) Plot of EMT marker E-cadherin, vimentin, and Snai1 in CRSwNPs and healthy control. (D) The relative mRNA expression of BRD4 after qRT-PCR analysis in primary hNPDECs and hNECs isolated and cultured from NP tissues (n = 7) and healthy nasal inferior turbinate tissues (n = 7). Data were expressed as mean ± SD. The Student test was used for statistical comparison. *P < 0.05, **P < 0.01 vs. controls.