FIGURE 1.

Aging mice with specific deletion of TTP in T cells develop chronic skin inflammation. (A) Growth curves of CD4^CreTTP^f/f mice and wild-type (WT) littermates were monitored and recorded weekly. Data are presented as mean ± SD of 6 mice in each group. (B) Data represent weight (g) of spleens and LN (means ± SD) from three CD4^CreTTP^f/f mice and WT littermate at 11–13 months old. Inserted images were spleen and lymph node of CD4^CreTTP^f/f mice and WT littermates. (C) Percentages of dermatitis. Five mice in each group. (D) Skin sections from CD4^CreTTP^f/f mice and WT mice were analyzed with hematoxylin and eosin staining. Cells from draining lymph nodes (LN) of the affected skin in five WT and CD4^CreTTP^f/f mice were stimulated with PMA and ionomycin for 4 h and analyzed by flow cytometry for intracellular cytokine gated on CD4⁺ T cells (E), or stimulated with anti-CD3 (1 μg/mL) with or without anti-CD28 (1 μg/mL) Abs for 3 days for detection of IL-17A by ELISA (F). Data shown are means ± SD from five mice. UD, undetectable. *p < 0.05, **p < 0.01, and ***p < 0.001 between groups.