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. 2020 Aug 14;11:1851. doi: 10.3389/fimmu.2020.01851

Figure 2.

Figure 2

CD107a expression of peripheral blood mononuclear cells (PBMCs) and whole blood (WB) under various cytokine combinations and incubation times. PBMCs or WB samples were mixed with media containing various cytokine combinations (no cytokines (media), IL-2, IL-2/IL-18, IL-2/IL-21, and IL-2/IL-18/IL-21) for the indicated durations of time, and CD107a assays were performed either immediately or following overnight incubation. Samples were incubated for 3 h with or without K562 cells. (A) Gating strategy of CD107a measurement. CD107a surface expression was determined as CD3−CD56+CD107a+ by flow cytometry. (B,C) Bar graphs indicate the percentage of CD107a+ NK cells in PBMCs following cytokine stimulation for 3 h or overnight. (D,E) Bar graphs indicate percentages of CD107a+ NK cells in WB following cytokine stimulation for 3 h and overnight. Data are represented as the mean ± SEM of results from five donors (*p < 0.05; **p < 0.01; ***p < 0.001).