Figure 3.

Comparison of NK cytotoxicity between the 4-h peripheral blood mononuclear cell (PBMC) assay without cytokine stimulation and the overnight whole blood (WB) assay with multiple cytokine combinations. PBMCs or WB were co-incubated with K562 cells, with various cytokine combinations added to WB, for 4 h (PBMCs) or overnight (WB). PBMC assays were performed at an E:T ratio of 40:1; WB assays used 200 μL WB. (A) Scatter plots of NK cytotoxicity in PBMCs and WB samples from 28 donors. Data are represented as the mean ± SEM (*p < 0.05; ***p < 0.001). (B) Representative data from two donors each with low, medium, and high NK cell cytotoxicity.