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. 2020 Aug 14;11:930. doi: 10.3389/fgene.2020.00930

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Copyright © 2020 Zhang, Zhang, Guan, Huang, Kong, Huang, Wang and Yang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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PCBP1 enhance p62 expression in multiple levels. (A) Immunoblots of p62 and LC3B in the indicated GFP-PCBP1 cells as well as their parental cells treated without (Control) or with Actinomycin D (ActD) or MG132, respectively. (B) Semi-quantitative RT-PCR analysis of p62 mRNA stability (upper panel) in A2780 cells with endogenous PCBP1 knockdown (KD) or PCBP1 overexpression (PCBP1), compared with control cells (GFP) by at three independents. GAPDH is used as an internal control, and the ratio of p62 mRNA level to GAPDH was quantified, normalized and indicated under each lane. *P < 0.05. (C) Immunoblot of p62 protein level in DLD-1 cells with or without ATG5 knockdown by at least two independents. GAPDH was used as an internal loading control.