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. 2020 Aug 14;11:1833. doi: 10.3389/fimmu.2020.01833

Figure 3.

Figure 3

Expression of complement factor C3 and CFH is different in cultured podocytes and glomerular endothelial cells. Conditionally immortalized human podocytes (HPC) and human glomerular endothelial cells (CiGenC) were compared for their expression of C3 and CFH on mRNA- and protein-level and secretion of both proteins. (A) mRNA-expression of C3/18S and (B) CFH/18S in HPC (white bars) and CiGenC (black bars) (n = 12). Protein expression of C3 and CFH was analyzed in Western blot (C) of whole cell lysates. Densitometry of C3 (D) and CFH (E) in HPC (white bars) and CiGenC (black bars) normalized to expression of beta (b)-actin (n = 6). Secretion of C3 and CFH into cell culture supernatant was tested in similar numbers of seeded cells and analyzed in Western Blot (F). Western blot densitometry of C3 (G) and CFH (H) from HPC-supernatants (white bars) and CiGenC-supernatants (black bars) (n = 6) Recombinant C3 and CFH were used as positive controls (Pos-Ctrl) in (C,F) in dilution 1:1,000. Data (relative expression) are shown in mean and SD in (A,B,D,E,G,H), **p < 0.01, ***p < 0.001, Mann Whitney U-test.