Fig. 2.

Synovial cell migration was inhibited by BAFF-siRNA. (A-C) MH7A cells were transfected with BAFF-siRNA. Then, 40,000 cells were plated to each ‘insert’ and allowed to migrate for 24 h. Then, cells were fixed, stained, and washed with water to remove redundant staining. Cells migrated onto lower part of ‘insert’ membrane were photographed (A), counted and presented as bar graph (B) after cells on upper part of ‘insert’ membrane were wiped out. Data were the representative of four experiments (A-C). Data in the bar graph represent the means ± SED. **p<0.01, significant difference as compared to scambled siRNA-treated control. RNA was isolated with Trizol. BAFF transcripts were measured by RT-PCR (C). (D) Chemical structure of KR-33426, [2-(2,5-dichlorophenyl)-5-methyloxazol-4yl carbonyl] guanidine.